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Rabbit Anti-CD90?antibody (bs-0778R)
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说明书: 50ul??100ul??200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包装/询价

产品编号 bs-0778R
英文名称 CD90
中文名称 CD90亚博国际亚博国际
别????名 CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.??
Specific References??(12)?????|?????bs-0778R has been referenced in 12 publications.
[IF=2.94]?Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro." Brain Research Bulletin (2013).??FCM?;??Rat.??
[IF=2.88]?Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).??Rat.??
[IF=2.26]?Yu, Xian-huan, et al. "Clinicopathological characteristics of 20 cases of hepatocellular carcinoma with bile duct tumor thrombi." Digestive diseases and sciences 56.1 (2011): 252-259.??IHC-P?;??Human.??
[IF=6.72]?Chen, Guobao, et al. "3D Scaffolds with Different Stiffness but Same Microstructure for Bone Tissue Engineering." ACS Applied Materials & Interfaces (2015).??IHC-F?;??Rat.??
[IF=1.52]?Tepekoy, Filiz, et al. "CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development." Reproductive Biology(2015).??IHC-P?;??Mouse.??
[IF=3.36]?Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).??Rat.??
[IF=1.80]?Vansandt, Lindsey M., et al. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology(2016).??IHC-P?;??Other Species.??
[IF=1.43]?Zeng, Biao, et al. "Increased expression of importin13 in endometriosis and endometrial carcinoma." Medical Science Monitor 18.6 (2012): CR361-CR367.??IHC-P?;??Human.??
[IF=1.06]?Scott, Erin M., et al. "Early histopathologic changes in the retina and optic nerve in canine primary angle‐closure glaucoma." Veterinary ophthalmology16.s1 (2013): 79-86.??IHC-P?;??Dog.??
[IF=0.00]?Gstraunthaler, Gerhard, et al. "Human platelet lysates successfully replace fetal bovine serum in adipose-derived adult stem cell culture." Journal of Advanced Biotechnology and Bioengineering 2.1 (2014): 1-11.??IF(ICC)?;??Human.??
[IF=0.52]?Utomo, Pamudji, et al. "Decreasing SDF1-CXCR4 Expression after Adipose-Derived Mesenchymal Stem Cells (ASCS) Treatment Combined with Freeze-Dried Amniotic Membrane Wrapping in Rat Sciatic Nerve Injury." International Journal of ChemTech Research.??IF(ICC)?;??Rat.??
[IF=1.92]?Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).??FCM?;??Sheep.??
研究领域 细胞生物??免疫学??神经生物学??
亚博国际亚博国际来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,?Mouse,?Rat,?Dog,?Pig,?Horse,?Sheep,?
产品应用 WB=1:500-2000?IHC-P=1:100-500?IHC-F=1:100-500?Flow-Cyt=1μg/Test?IF=1:100-500?(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量 12kDa
细胞定位 细胞膜?
性????状 Lyophilized or Liquid
浓????度 1mg/ml
免?疫?原 KLH conjugated synthetic peptide derived from rat Thy-1:31-120/161?
亚????型 IgG
纯化方法 affinity purified by Protein A
储?存?液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Abundant in lymphoid tissues.

Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01830

Gene ID:
24832

Database links:

Entrez Gene: 7070?Human

Entrez Gene: 21838?Mouse

Entrez Gene: 24832?Rat

Omim: 188230?Human

SwissProt: P04216?Human

SwissProt: P01831?Mouse

SwissProt: P01830?Rat

Unigene: 644697?Human

Unigene: 3951?Mouse

Unigene: 108198?Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Thy-1/CD90/ Thy1.1 Polyclonal Antibody, Unconjugated(bs-0778R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-CD90 antibody(bs-0778R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-0778R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti- CD90 antibody (bs-0778R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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